Integrating Multi-Omics for Uncovering the Architecture of Cross-Talking Pathways in Breast Cancer

Cross-talk among abnormal pathways widely occurs in human cancer and generally leads to insensitivity to cancer treatment. Moreover, alterations in the abnormal pathways are not limited to single molecular level. Therefore, we proposed a strategy that integrates a large number of biological sources at multiple levels for systematic identification of cross-talk among risk pathways in cancer by random walk on protein interaction network. We applied the method to multi-Omics breast cancer data from The Cancer Genome Atlas (TCGA), including somatic mutation, DNA copy number, DNA methylation and gene expression profiles. We identified close cross-talk among many known cancer-related pathways with complex change patterns. Furthermore, we identified key genes (linkers) bridging these cross-talks and showed that these genes carried out consistent biological functions with the linked cross-talking pathways. Through identification of leader genes in each pathway, the architecture of cross-talking pathways was built. Notably, we observed that linkers cooperated with leaders to form the fundamentation of cross-talk of pathways which play core roles in deterioration of breast cancer. As an example, we observed that KRAS showed a direct connection to numerous cancer-related pathways, such as MAPK signaling pathway, suggesting that it may be a central communication hub. In summary, we offer an effective way to characterize complex cross-talk among disease pathways, which can be applied to other diseases and provide useful information for the treatment of cancer.     

编者按

正《2021年阿尔茨海默病事实和数据》(Alzheimer's Association)强调了阿尔茨海默病(Alzheimer's disease,AD)是最常见的痴呆症.伴随人类老龄化的日益严重,AD已被列为美国的第六大死因,在我国已成为第五大死因,且死亡率呈逐年上升趋势.《2019年世界阿尔茨海默病报告:对痴呆症的态度》估计全球有超过5 000万人患有痴呆症,预计到2050年,这个数字可能增加到1.52亿[1].     

Functionally distinct roles for TET-oxidized 5-methylcytosine bases in somatic reprogramming to pluripotency

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Circular RNA WHSC1 exerts oncogenic properties by regulating miR-7/TAB2 in lung cancer

Circular RNA is a newly discovered member of non-coding RNA (ncRNA) and regulates the target gene by acting as a micro-RNA sponge. It plays vital roles in various diseases. However, the functions of circular RNA in non-small cell lung cancer (NSCLC) remain still unclear. Our data showed that circ-WHSC1 was highly expressed in NSCLC cells and tissues. Both in vitro and in vivo experiments showed that circ-WHSC1 promoted NSCLC proliferation. circ-WHSC1 also promoted the migration and invasion of lung cancer cells. Through bioinformatic analysis and functional experiments, we showed that circ-WHSC1 could act as a sponge for micro-RNA-7 (miR-7) and regulate the expression of TAB2 (TGF-beta activated kinase one binding protein two). Inhibition of the circ-WHSC1/miR-7/TAB2 pathway could effectively attenuate lung cancer progression. In summary, this study confirmed the existence and oncogenic function of circ-WHSC1 in NSCLC. The research suggests that the circ-WHSC1/miR-7/TAB2 axis might be a potential target for NSCLC therapy.     

高穗花报春的组织培养和快速繁殖

1植物名称高穗花报春(Primula vialii Franch.). 2材料类别种子萌发的无菌苗上胚轴. 3培养条件(1)种子萌发培养基:MS;(2)不定芽诱导和增殖培养基:MS NAA 0.2 mg·L-1(单位下同) IBA 0.5 6-BA 0.5;(3)生根培养基:1/2MS IBA 1.0.培养基中均附加3%蔗糖、0.8%琼脂,pH 5.8,在121℃下高压灭菌15 min.培养温度为(25±2)℃,光照时间为12 h·d-1,光强为80 μmol·m-2·s-1.     

球孢白僵菌对四种十字花科蔬菜害虫的兼控潜力评价

研究了分离自小猿叶甲的一株球孢白僵菌(Beauveria bassiana(SCAU-BB01D))对十字花科蔬菜4种非目标害虫烟粉虱Bemisia tabaci、小菜蛾Plutella xylostella、黄曲条跳甲Phyllotreta striolata和菜缢管蚜Lipaphis erysimi的致病能力。结果表明,在实验室条件下,该菌株对以上4种害虫均有不同程度的侵染力。1×108孢子/mL的白僵菌悬浮液对1,2,3,4龄烟粉虱若虫的侵染率分别为84.88,86.81,55.94和38.78%;对小菜蛾2,3和4龄幼虫的侵染率为67,59和44%;对黄曲条跳甲幼虫和成虫分别为63%和60%;对菜缢管蚜成蚜和若蚜的侵染率分别为44%和35%。通过机率值分析,得出该菌株对烟粉虱1~4龄若虫的LT50分别为4.14,3.78,6.24和7.59 d;对小菜蛾2~4龄幼虫的LT50分别为7.29,8.16和9.82d;对黄曲条跳甲成虫和幼虫的LT50分别为11.22和8.11 d;对菜缢管蚜成蚜和若蚜的LT50分别为11.01和12.15 d。说明该球孢白僵菌菌株对烟粉虱、小菜蛾和黄曲条跳甲3种非目标害虫具有一定的兼控作用,在蔬菜害虫的生物防治中具一定的应用潜力。     

不同无性系蔗茅抽穗开花特性的观察和花粉贮藏条件的研究

来源于较低海拔地区的3个蔗茅无性系的抽穗期较短,来源于较高海拔(大于1500m)地区的16个蔗茅无性系的抽穗期较长,且抽穗期与原产地海拔呈显著负相关(F=-0.827**).蔗茅的这一特性在集中种植后仍能相对稳定地表现出来.蔗茅花粉量较多,花粉发育良好.在自然条件下,蔗茅新鲜花粉仅能存活1 h左右;而经低温(10℃)硅胶干燥2 h后贮藏于-80℃条件下,其生活力保持可超过360d.蔗茅贮藏花粉生活力的下降比率与花粉含水量呈显著正相关(r=0.933**);含水量降至10%以下的花粉在低温中贮藏时才能保持较高生活力.     

赤眼蜂田间混合种群的分离、鉴定及其性比的确定

介绍了一种适用于赤眼蜂Trichogramma田间混合种群分离、鉴定及性比确定的简单而有效的方法,并据此方法成功地鉴定出4种自然寄生于小菜蛾卵的寄生蜂种类,拟澳洲赤眼蜂TrichogrammaconfusumViggiani、玉米螟赤眼蜂Trichogramma.ostriniaePangetChen、微突赤眼蜂TrichogrammaraioNagaraja及卷蛾分索赤眼蜂TrichogrammatoideabactraeNagaraja。     

饲养黄曲条菜跳甲实验种群的一种新方法

介绍一种简便可行的饲养黄曲条菜跳甲Phyllotretastriolata(F.)的新方法———整苗大瓶罩养法。用5种植物根部饲养越冬代,黄曲条菜跳甲均可成功地完成其生活周期,未成熟期最高存活率为63.3%,成虫平均寿命最长为219.2d,平均产卵量最大为192.4粒雌。